Spermatogonial Cell Proliferation in Organ Culture of Immature Rat Testis1
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چکیده
منابع مشابه
Increase of Proliferation and Colonization in Mouse Spermatogonial Stem Cell by Low Intensity Ultrasound
Background Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. Sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study we investigated the effect of LIUS stimulation on mouse SSCs proliferation and colonization. Methods: Isolated SSCs from neonate mice cultured in DMEM culture medium with 10% Fetal Bovine S...
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Methods are detailed for isolating highly pure populations of spermatogonial stem cells from primary cultures of testis cells prepared from 22- to 24-day-old rats. The procedure is based on the principle that testicular somatic cells bind tightly to plastic and collagen matrices when cultured in serum-containing medium, whereas spermatogonia and spermatocytes do not bind to plastic or collagen ...
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Background: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells have provided a very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. To investigate and biomanipulate these cells, first the proliferation and viability rate of cells s...
متن کاملReconstitution of mouse spermatogonial stem cell niches in culture.
Spermatogonial stem cells (SSCs) reside in specific niches within seminiferous tubules. These niches are thought to secrete chemotactic factors for SSCs, because SSCs migrate to them upon transplantation. However, the identity of these chemotactic molecules remains unknown. Here, we established a testis feeder cell culture system and used it to identify SSC chemotactic factors. When seeded on t...
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Background: While articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. Several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. Methods: Rat articu...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1993
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod48.4.761